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GrainGenes Marker Report: Xcfa2226b

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Locus	Xcfa2226b
Type	Microsatellite
Chromosome	1A
Map	Wheat-Composite2004-1A 31
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Map Data	Wheat, Composite, 2004
Probe	CFA2226
Possible Orthologs	cfa2226 Xcfa2226-1A Xcfa2226-3B cfa2226-1A
Probe for Locus	CFA2226
Locus	Xcfa2226-1A Xcfa2226-3B Xcfa2226b cfa2226 cfa2226-1A
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GCT)6 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GGAGAAAAGCAAACAGCGAC 3' Reverse 5' CAGTAGCATCTTCCATGGCG 3'
SSR size	196
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3B/1A
Source Species	Triticum urartu