GrainGenes Marker Report: Xcfa2219-1A
Locus
Xcfa2219-1A
Type
Microsatellite
Chromosome
1A
Map
Chinese_Spring_Deletion_SSR_1A 0.5 Kofa/UC1113-1A 118 Ta-Courtot/CS-GPW-1A 153.8 Ta-SSR-2004-1A 124.1
[ Show Nearby Loci ]
Breakpoint Interval
C-1AL-0.00-1.00*
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe
CFA2219
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2219 cfa2219-1A cfa2219
Probe for Locus
CFA2219
Locus
Xcfa2219-1A Xcfa2219 cfa2219-1A cfa2219
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GT)21 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TCTGCCGAGTCACTTCATTG 3'
Reverse 5' GACAAGGCCAGTCCAAAAGA 3'
SSR size
223
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2219-1A
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