GrainGenes Marker Report: Xcfa2201-2B
Locus
Xcfa2201-2B
Type
Microsatellite
Map
Kofa/UC1113-2B 17.3
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113
Probe
CFA2201
Possible Orthologs
Xcfa2201 cfa2201-2A cfa2201 cfa2201-2B
Probe for Locus
CFA2201
Locus
Xcfa2201-2B Xcfa2201 cfa2201-2A cfa2201 cfa2201-2B
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)14 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' CAAACCAACCTCATTGACCC 3'
Reverse 5' CCACCAGAACTTCAACCTGG 3'
SSR size
216
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2201-2B
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