GrainGenes Marker Report: Xcfa2191-3B
Locus
Xcfa2191-3B
Map
Ta-Synthetic/Opata-GPW-3B -46.8
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Map Data
Wheat, Synthetic x Opata, GPW
Probe
CFA2191
Probe for Locus
CFA2191
Locus
Xcfa2191-3B cfa2191
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TCCC)4 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' AGAGCAGGAGGTTGGGTTCT 3'
Reverse 5' CCGGAATTTCACTACCAGGA 3'
SSR size
162
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3B
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2191-3B
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