GrainGenes Marker Report: Xcfa2185-5A
Locus
Xcfa2185-5A
Type
Microsatellite
Chromosome
5A
Map
Kofa/UC1113-5A 166.1 Ta-SSR-2004-5A 113.5
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004
Probe
CFA2185
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2185-5D Xcfa2185.1 Xcfa2185.2 cfa2185 cfa2185-5A
Probe for Locus
CFA2185
Locus
Xcfa2185-5D
[ Show all 6 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)29 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TTCTTCAGTTGTTTTGGGGG 3'
Reverse 5' TTTGGTCGACAAGCAAATCA 3'
SSR size
226
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5D
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2185-5A
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