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GrainGenes Marker Report: Xcfa2174

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Locus	Xcfa2174
Type	Microsatellite
Map	Ta-NxW-7D 113.2
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	CFA2174
Possible Orthologs	Xcfa2174-4A [ Show all 8 ]
Probe for Locus	CFA2174
Locus	Xcfa2174-4A [ Show all 9 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)15(GT)14 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' ACGGCATCACAGGTTAAAGG 3' Reverse 5' GGTCTTTGCACTGCTAGCCT 3'
SSR size	191
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7A/3D/7D
Source Species	Triticum urartu