GrainGenes Marker Report: Xcfa2173-4D
Locus
Xcfa2173-4D
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_4D 0.855 Ta-Synthetic/Opata-GPW-4D 27.1
[ Show Nearby Loci ]
Breakpoint Interval
4DL12-0.71-1.00
Map Data
Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFA2173
Possible Orthologs
Xcfa2173 Xcfa2173-4A Xcfa2173-6A Xcfa2173b
Probe for Locus
CFA2173
Locus
Xcfa2173-4A Xcfa2173-4D Xcfa2173 Xcfa2173b Xcfa2173-6A
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)28 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GACATACTCCGGCGTTGAAT 3'
Reverse 5' TTCCCAGGACATCCTTCTTG 3'
SSR size
258
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
?/4D
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2173-4D
|
| ||||||
|
| ||||||
|
| ||||||
| |||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
|