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GrainGenes Marker Report: Xcfa2164

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Locus	Xcfa2164
Type	Microsatellite
Map	Svevo/Ciccio-2A 98.9
	[ Show Nearby Loci ]
Breakpoint Interval	C-2AS5-0.78
Map Data	Durum wheat, Svevo x Ciccio
Probe	CFA2164
Possible Orthologs	Xcfa2164-2A [ Show all 10 ]
Probe for Locus	CFA2164
Locus	Xcfa2164b-2A [ Show all 11 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)8(GA)9 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GGGTTGGTGCCTAGATTGAA 3' Reverse 5' TCAAGGTGCCAACACTTACG 3'
SSR size	295
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2A/3A
Source Species	Triticum urartu