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GrainGenes Marker Report: Xcfa2158b

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Locus	Xcfa2158b
Type	Microsatellite
Chromosome	1B
Chromosome Arm	1BS
Map	Tt-LxG-1B 27.1 Wheat-Composite2004-1B 21
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Map Data	T. turgidum, Langdon x G18-16 Wheat, Composite, 2004
Probe	CFA2158
Possible Orthologs	Xcfa2158-1A [ Show all 13 ]
Probe for Locus	CFA2158
Locus	Xcfa2158a [ Show all 14 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)21 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TTTCGTCTTCAAAATGCACTG 3' Reverse 5' TGGTAGCTTACAAAGGTGCG 3'
SSR size	229
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1A/1B/1D
Source Species	Triticum urartu