GrainGenes Marker Report: Xcfa2158-1B
Locus
Xcfa2158-1B
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_1B 0.16 Ta-Courtot/CS-GPW-1B 30.2
[ Show Nearby Loci ]
Breakpoint Interval
C-1BL6-0.32
Map Data
Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe
CFA2158
Possible Orthologs
Xcfa2158-1A
[ Show all 13 ]
Probe for Locus
CFA2158
Locus
Xcfa2158a
[ Show all 14 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)21 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TTTCGTCTTCAAAATGCACTG 3'
Reverse 5' TGGTAGCTTACAAAGGTGCG 3'
SSR size
229
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A/1B/1D
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2158-1B
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