GrainGenes Marker Report: Xcfa2153-1A.1
Locus
Xcfa2153-1A.1
Type
Microsatellite
Map
USG3209/Jaypee-1A 17.6
[ Show Nearby Loci ]
Map Data
Wheat, USG3209 x Jaypee
Probe
CFA2153
Possible Orthologs
Xcfa2153 Xcfa2153-1A Xcfa2153-1A.2
Probe for Locus
CFA2153
Locus
Xcfa2153-1A Xcfa2153-1A.1 Xcfa2153-1A.2 Xcfa2153 H9/H10/H11
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)27 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TTGTGCATGATGGCTTCAAT 3'
Reverse 5' CCAATCCTAATGATCCGCTG 3'
SSR size
200
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2153-1A.1
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