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GrainGenes Marker Report: Xcfa2135-1A

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Locus	Xcfa2135-1A
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_1A 0.5 Ta-Courtot/CS-GPW-1A 53.1
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Breakpoint Interval	C-1AL-0.00-1.00*
Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe	CFA2135
Possible Orthologs	Xcfa2135
Probe for Locus	CFA2135
Locus	Xcfa2135-1A Xcfa2135
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA23) Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TGCCTAAATCTAAATGCCCG 3' Reverse 5' GGATAATGTGCATGTTCACCG 3'
SSR size	175
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1A
Source Species	Triticum urartu