GrainGenes Marker Report: Xcfa2134-3A
Locus
Xcfa2134-3A
Type
Microsatellite
Chromosome
3A
Chromosome Arm
3AL
Map
T.b.xT.m.-3A 103.2 Ta-Courtot/CS-GPW-3A 97.8 Ta-SSR-2004-3A 50.5 Triticum-AxF-3A 80.5
[ Show Nearby Loci ]
Map Data
T. boeoticum x monococcum Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW
Species
Triticum aestivum
Probe
CFA2134
Reference
Possible Orthologs
cfa2134 Xcfa2134 Xcfa2134-3B cfa2134-3B cfa2134-3A
Probe for Locus
CFA2134
Locus
Xcfa2134-3A
[ Show all 6 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TC)n Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TTTACGGGGACAGTATTCGG 3'
Reverse 5' AAGACACTCGATGCGGAGAG 3'
SSR size
210
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2134-3A
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