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GrainGenes Marker Report: Xcfa2129-1D

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Locus	Xcfa2129-1D
Type	Microsatellite
Chromosome	1D
Map	Louise/Penawawa-1D 0 P91193D1/P92201D5-1D 77.6 Ta-SSR-2004-1D 73
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, P91193D1 x P92201D5
Probe	CFA2129
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfa2129 [ Show all 14 ]
Probe for Locus	CFA2129
Locus	Xcfa2129-1A [ Show all 15 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)25 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GTTGCACGACCTACAAAGCA 3' Reverse 5' ATCGCTCACTCACTATCGGG 3'
SSR size	159
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1A
Source Species	Triticum urartu