GrainGenes Marker Report: Xcfa2129-1B
Locus
Xcfa2129-1B
Type
Microsatellite
Chromosome
1B
Map
Kofa/UC1113-1B 84.3 Ta-SSR-2004-1B 39.3
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004
Probe
CFA2129
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2129
[ Show all 14 ]
Probe for Locus
CFA2129
Locus
Xcfa2129-1A
[ Show all 15 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)25 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GTTGCACGACCTACAAAGCA 3'
Reverse 5' ATCGCTCACTCACTATCGGG 3'
SSR size
159
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2129-1B
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