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GrainGenes Marker Report: Xcfa2106-7B

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Locus	Xcfa2106-7B
Type	Microsatellite
Chromosome	7B
Map	Ta-SSR-2004-7B 37.9 Ta-Synthetic/Opata-GPW-7B 7.4
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Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW
Probe	CFA2106
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	cfa2106
Probe for Locus	CFA2106
Locus	Xcfa2106-7B cfa2106
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CCG)5 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GCTGCTAAGTGCTCATGGTG 3' Reverse 5' TGAAACAGGGGAATCAGAGG 3'
SSR size	159
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7B
Source Species	Triticum urartu