GrainGenes Marker Report: Xcfa2099-7D
Locus
Xcfa2099-7D
Type
Microsatellite
Map
Ta-Courtot/CS-GPW-7D 153.2
[ Show Nearby Loci ]
Map Data
Wheat, Courtot x Chinese Spring, GPW
Probe
CFA2099
Possible Orthologs
Xcfa2099-2A Xcfa2099a Xcfa2099b
Probe for Locus
CFA2099
Locus
Xcfa2099-2A Xcfa2099-7D Xcfa2099a Xcfa2099b
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)24 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TGCGAAGTATTCAGTGCGTC 3'
Reverse 5' TCAAGACCATCAGCACTCAGA 3'
SSR size
277
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2A/7D
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2099-7D
|
| |||||
|
| |||||
|
| |||||
| ||||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
|