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GrainGenes Marker Report: Xcfa2056b

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Locus
Xcfa2056b
Type
Microsatellite
Chromosome
1A
Map
Wheat-Composite2004-1A96
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Map Data
Wheat, Composite, 2004
Probe
CFA2056
Possible Orthologs
Xcfa2056-1A
Xcfa2056-7A



Probe for Locus
CFA2056
Locus
Xcfa2056-1A
Xcfa2056-7A
Xcfa2056b
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = D
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CGC)4
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TGGAAGTTACCCTGTCCGTC 3'
Reverse 5' GAAGAGAGGAGGAGTGGCCT 3'
SSR size
268
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A/1A
Source Species
Triticum urartu