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GrainGenes Marker Report: Xcfa2037-3A

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Locus
Xcfa2037-3A
Type
Microsatellite
Map
Ta-Courtot/CS-GPW-3A204.7
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Map Data
Wheat, Courtot x Chinese Spring, GPW
Probe
CFA2037
Possible Orthologs
Xcfa2037



Probe for Locus
CFA2037
Locus
Xcfa2037-3A
Xcfa2037
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = C
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CA)23
Regularity of motif is imperfect
Type
PCR
SSR
PCR primers
Forward 5' CCCCTTTTGGTCTGCTGTAA 3'
Reverse 5' CAGTGTGCCTTCTAGCCCTC 3'
SSR size
206
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3D
Source Species
Triticum urartu