GrainGenes Marker Report: Xcfa2026-4A
Locus
Xcfa2026-4A
Type
Microsatellite
Map
Ta-Courtot/CS-GPW-4A 25.6
[ Show Nearby Loci ]
Map Data
Wheat, Courtot x Chinese Spring, GPW
Probe
CFA2026
Probe for Locus
CFA2026
Locus
Xcfa2026-4A
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)24 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' CTAATCTAAGCAAGGGCCG 3'
Reverse 5' TTACTGCACTGCGAGTTGCT 3'
SSR size
233
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2026-4A
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