GrainGenes Marker Report: Xcfa2019-7A
Locus
Xcfa2019-7A
Type
Microsatellite
Chromosome
7A
Map
Chinese_Spring_Deletion_SSR_7A 0.88 T.b.xT.m.-7A 253.9 Ta-Courtot/CS-GPW-7A 224 Ta-SSR-2004-7A 106.6
[ Show Nearby Loci ]
Breakpoint Interval
7AL16-0.86-0.90
Map Data
T. boeoticum x monococcum Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe
CFA2019
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
cfa2019 Xcfa2019
Probe for Locus
CFA2019
Locus
Xcfa2019-7A Sr22 cfa2019 Xcfa2019
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)14 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GACGAGCTAACTGCAGACCC 3'
Reverse 5' CTCAATCCTGATGCGGAGAT 3'
SSR size
217
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2019-7A
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