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GrainGenes Marker Report: WMS654

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Probe	WMS654
Locus	Xgwm654-5D Xgwm654 gwm654
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Remarks	Insert contains repeat (GT)28 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGCTGATGTTGTAAGAAGGC 3' 5' TGCGTCAGATATGCCTACCT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS654
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring