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GrainGenes Marker Report: WMS577

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Probe	WMS577
Locus	Xgwm577-7B [ Show all 12 ]
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Remarks	Insert contains repeat (CA)14(TA)6 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. In 369 Avena sterilis accessions there were 2 alleles of size 50-55 bp, PIC = negative.
Type	Genomic PCR SSR
PCR primers	5' ATGGCATAATTTGGTGAAATTG 3' 5' TGTTTCAAGCCCAACTTCTATT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS577
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring