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GrainGenes Marker Report: WMS359

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Probe	WMS359
Locus	Xgwm359-2A Xgwm359 YrR61 gwm359
Reference	Hao Y et al. (2011) Characterization of a major QTL for adult plant resistance to stripe rust in US soft winter wheat. Theoretical and Applied Genetics 123:1401-1411. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. YrR61 MAS Wheat. Marker Assisted Selection in Wheat.
Remarks	Insert contains repeat (CT)20(CTT)13imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CTAATTGCAACAGGTCATGGG 3' 5' TACTTGTGTTCTGGGACAATGG 3' WMS359-F 5'- AGC CGC GAA ATC TAC TTT GA WMS359-R 5'- TTA AAC GGA CAG AGC ACA CG
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C Denaturing step: 94C, 3 min. Touchdown step (decrease 1C/cycle for 15 cycles): 94C, 45 sec; 65-50C, 50 sec; 72C, 55 sec. Amplification step (30 cycles): 95C, 40 sec; 50C, 40 sec; 72C, 40 sec. Extensions step: 72C, 5 min
Polymorphism	WMS359
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring