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GrainGenes Marker Report: WMC443

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Probe	WMC443
Locus	Xwmc443 Xwmc443-5D wmc443 wmc443-5D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24a7 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 205 bases located on 5D Trimmed to insert at 5' end and to Ssp1 at 3' end. (CT)44 62 to 149.
Type	PCR SSR
PCR primers	Forward ccTccTcTgTTTTcccTcTgTT at 14 length 22 bases, Tm 63 Reverse cAcAcTcTgTgcTTcTgTTTgc at 198 length 22 bases, Tm 63
SSR size	206 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC443
Source Species	Triticum aestivum