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GrainGenes Marker Report: WMC307

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Locus	wmc307
Type	Microsatellite
Map	Physical-3B 64.43 Wheat, Yr genes and QTL 3B 42.3 Wheat-2014-Consensus-Ug99-Update_3B 34.77 Wheat_Consensus_2014_Ug99_Rust_3B 34.77 Wheat_Yr_2021-3B 90.2
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Map Data	3B Physical Wheat, Yr genes and QTL Wheat, Yr Update, 2021 Wheat-2014-Consensus-Ug99-Update Wheat_Consensus_2014_Ug99_Rust
Probe	WMC307
Possible Orthologs	Xwmc307-3B wmc307-3B
Probe	WMC307
Locus	Xwmc307-3B wmc307-3B wmc307
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8c5 Donor: Rouse, Novartis Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 146 bases located on 3B Contains (GT)8(GA)13. Trimed to Rsa1 at 5' end and to insert at 3 ' end. (GA)13 171 to 196, (GT)8 155 to 170.
Type	PCR SSR
PCR primers	Forward gTTTgAAgAccAAgcTccTccT at 81 length 22 bases, Tm 63 Reverse AccATAAccTcTcAAgAAcccA at 204 length 22 bases, Tm 62
SSR size	145 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC307
Source Species	Triticum aestivum