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GrainGenes Marker Report: WMC121

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Probe	WMC121
Locus	Xwmc121-7D Xwmc121 wmc121
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8e12 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 309 bases located on 7D AGR200. Trimmed to insert at 5' end and to Rsa1 site at 3' end. (GA)12 207 to 230. Maybe polymorphic.
Type	PCR SSR
PCR primers	Forward ggcTgTggTcTcccgATcATTc at 26 length 22 bases, Tm 69 Reverse AcTggAcTTgAggAggcTggcA at 312 length 22 bases, Tm 69
SSR size	308 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC121
Source Species	Triticum aestivum