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GrainGenes Marker Report: Stb13

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Locus
Stb13
Type
Trait
Chromosome
7B
Chromosome Arm
7BL
Map
Wheat-Composite2004-7B60
[ Show Nearby Loci ]
Species
Triticum aestivum
Probe
WMC396
Associated Gene
Stb13 (Triticum)
Gene Class
Reaction to Mycosphaerella graminicola (Fuckel) Schroeter
Remark
Trait marker Xwmc396-7B
Nearby marker on Composite map: Xwmc396
Position on Composite map estimated by GrainGenes.



Probe for Locus
WMC396
Locus
Xwmc396-7B
Xwmc396
Stb13
wmc396-7B
wmc396
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10g12
Donor: Jacquemin, Gembloux
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 149 bases located on 7B
Trimmed to Rsa1 at 5' end, 3' end not located. (GT)14 122 to 149. Bases 73 to 250 show homology to 14h11 bases 1 to 178 (complement) (178 in longest run, maxScore = 119). Duplicate of 14h11, 10g12 has priority.
Type
PCR
SSR
PCR primers
Forward TgcAcTgTTTTAccTTcAcggA at 27 length 22 bases, Tm 65
Reverse cAAAgcAAgAAccAgAgccAcT at 151 length 22 bases, Tm 65
SSR size
146 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide.
Sequence
WMC396
Source Species
Triticum aestivum