GrainGenes Marker Report: Sr22
Locus
Sr22
Type
Gene Trait
Chromosome
7A
Chromosome Arm
7AL
Map
Triticum-Genes-7A 29.4 Wheat-2014-Consensus-Ug99-Update_7A 112 Wheat-Composite2004-7A 171
[ Show Nearby Loci ]
Map Data
Wheat genes Wheat-2014-Consensus-Ug99-Update
2 Point Data
Centromere/Sr22#1 Distance: 27 cM
[ Show all 7 ]
Species
Triticum aestivum
Probe
BARC121 CFA2019 CFA2123 WMC633
Associated Gene
Sr22 (Triticum)
Gene Class
Reaction to Puccinia graminis Pers.
Reference
Data Source
Matthews, David E.
Remark
Trait marker Xcfa2019-7A Nearby marker on Composite map: Xgwm282 Position on Composite map estimated by GrainGenes. The location on the 2014 Ug99 Consensus Map is approximate.
Probe for Locus
CFA2019
Locus
Xcfa2019-7A Sr22 cfa2019 Xcfa2019
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)14 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GACGAGCTAACTGCAGACCC 3'
Reverse 5' CTCAATCCTGATGCGGAGAT 3'
SSR size
217
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A
Source Species
Triticum urartu
GrainGenes Marker Report: Sr22
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