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GrainGenes Marker Report: Sr21

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Locus
Sr21
Type
Gene
Chromosome
2A
Chromosome Arm
2AL
Map
Triticum-Genes-2A4.7
Wheat-2014-Consensus-Ug99-Update_2A70
[ Show Nearby Loci ]
Map Data
Wheat genes
Wheat-2014-Consensus-Ug99-Update
2 Point Data
Centromere/Sr21#1Distance: 2.4 cM
Sr21/Pm4b#1Distance: 37.5 cM
Probe
EX594406
FD527726
WMS312
Associated Gene
Sr21 (Triticum)
Gene Class
Reaction to Puccinia graminis Pers.
Reference
ReferenceChen S et al. (2015) Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group Theoretical and Applied Genetics 128:645-656.
ReferenceYu L et al. (2014) A consensus map for Ug99 stem rust resistance loci in wheat. Theoretical and Applied Genetics 127:1561-1581.
ReferenceThe TT et al. (1979) Cytogenetical studies in wheat. IX. Monosomic analyses, telocentric mapping and linkage relationships of genes Sr21, Pm4, and Mle. Australian Journal of Biological Sciences 32:115-125.
ReferenceMAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr21 MAS Wheat. Marker Assisted Selection in Wheat.


Probe for Locus
WMS312
Locus
Xgwm312-2A
[ Show all 7 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)37
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' ATCGCATGATGCACGTAGAG 3'
5' ACATGCATGCCTACCTAATGG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS312
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring