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GrainGenes Marker Report: Rht4

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Locus	Rht4
Type	Trait
Chromosome	2B
Chromosome Arm	2BL
Map	Wheat-Composite2004-2B 125
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	WMC317
Associated Gene	Rht4 (Triticum)
Gene Class	Reduced height
Reference	Ellis MH et al. (2005) Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat Theoretical and Applied Genetics 111:423-430.
Remark	Trait marker Xwmc317-2B Nearby marker on Composite map: Xwmc317 Position on Composite map estimated by GrainGenes.
Probe for Locus	WMC317
Locus	Xwmc317 [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14f4 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 146 bases located on 2B Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)23 62 to 107. Bases 61 to 197 show homology to 15a5 bases 4 to 140 (complement) (137 in longest run, maxScore = 81). 14f4 is a duplicate of 15a5. 14f4 has priority.
Type	PCR SSR
PCR primers	Forward TgcTAgcAATgcTccgggTAAc at 6 length 22 bases, Tm 67 Reverse TcAcgAAAccTTTTccTccTcc at 123 length 22 bases, Tm 65
SSR size	139 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC317
Source Species	Triticum aestivum