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GrainGenes Marker Report: RG1

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Locus	Rg1
Type	Gene STS
Chromosome	1B
Chromosome Arm	1BS
Map	Triticum-Genes-1B -49 [ Show all 6 ]
	[ Show Nearby Loci ]
Map Data	T. turgidum, Messapia x dicoccoides [ Show all 6 ]
2 Point Data	Rg1/(Gli-B1)#1 Distance: 2 cM [ Show all 13 ]
Probe	Rg-B1
Associated Gene	Rg1 (Triticum)
Gene Class	Red glumes
Reference	Abrouk M et al. (2021) Population genomics and haplotype analysis in spelt and bread wheat identifies a gene regulating glume color Communications Biology 4.
Probe for Locus	Rg-B1
Locus	Rg1
Reference	Abrouk M et al. (2021) Population genomics and haplotype analysis in spelt and bread wheat identifies a gene regulating glume color Communications Biology 4.
General Remarks	Amplicon: Group 1,2,4,5: 381bp. Group 3: 334bp Amplification conditions: A touch-down PCR protocol was run as follows: initial denaturation at 94 degr. C for 30 seconds; annealing at 62 degr. C for 30 seconds, decreasing by 0.5 degr. C/cycle; extension at 72 degr. C for 45 seconds, followed by repeating these steps for eight cycles. After enrichment, the program continued for 29 cycles as follows: 94 degr. C for 30 seconds, 58 degr. C for 30 seconds and 72 degr. C for 45 seconds. Group 3 is associated with the red glume gene
Type	STS
PCR primers	CTCGCAAAATACATWGCTGG ATGSTGGAGTAATCCAAGGC
Sequence	Group_1,2,4,5_allele Group_3_allele
Source Species	Triticum aestivum
Source Germplasm	Jagger