GrainGenes Marker Report: Ne2
Locus
Ne2
Type
Gene Trait
Chromosome
2B
Chromosome Arm
2BS
Map
Triticum-Genes-2B -3.9 Wheat-Composite2004-2B 69
[ Show Nearby Loci ]
Map Data
Wheat genes
2 Point Data
Ne2/Centromere#1 Distance: 9.4 cM Ne2/D2#1 Distance: 34 cM
Species
Triticum aestivum
Probe
WMS148
Associated Gene
Ne2 (Triticum)
Gene Class
Hybrid necrosis
Reference
Chu CG et al. (2006) Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers. Theoretical and Applied Genetics 112:1374.
Remark
Trait marker Xgwm148-2B Nearby marker on Composite map: Xgwm148 Position on Composite map estimated by GrainGenes.
Probe for Locus
WMS148
Locus
Xgwm148-2B Xgwm148 Ne2 gwm148 gwm148-2B
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GTGAGGCAGCAAGAGAGAAA 3'
5' CAAAGCTTGACTCAGACCAAA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS148
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Ne2
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