GrainGenes Marker Report: MWG897
Locus
MWG897
Type
CAPS RFLP STS
Chromosome
6H
Chromosome Arm
6HL
Map
Barley-consensus-2003-6H 118.4
[ Show all 10 ]
[ Show Nearby Loci ]
Map Data
Barley consensus
[ Show all 10 ]
Species
Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe
MWG897
Reference
Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Data Source
Kleinhofs, Andris Langridge, Peter J.
Remark
This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Igri x Franka Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Igri x Franka
Possible Orthologs
Xmwg897 Xmwg897-6A Xmwg897-6B
Probe
MWG897
Locus
MWG897 Xmwg897-6B Xmwg897-6A Xmwg897
Reference
Remarks
Restriction Enzyme for CAPS marker is Dde I
Type
Genomic STS
PCR primers
5' ggatcagtgatttcgccgtgtttct 3'
5' ccatgatgcaaagctccaactactc 3'
STS primers
T7 side (5' to 3') GGATCAGTGATTTCGCCGTGTTTCT
T3 side (5' to 3') CCATGATGCAAAGCTCCAACTACTC
Amplification Conditions
5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 63 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence
AJ234632 AJ234631 MWG897 MWG897-5 MWG897-3
Linkage Group
6H
Insert Enzyme
PstI
Source Species
Hordeum vulgare
Insert Size
1.2
GrainGenes Marker Report: MWG897
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