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GrainGenes Marker Report: HvJASIP

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Locus	HvJASIP
Type	Microsatellite
Chromosome	3H 4H
Map	Hordeum-Graner2-4H 135.2
	[ Show Nearby Loci ]
Map Data	Barley, IxF
Probe	HvJASIP
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	HvJASIP
Locus	HvJASIP
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (acg)4 Polymorphic Information Content (PIC) = 0.4 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	Left 5' CGACGGCAACTACAACACGC Right 5' CCACTGCTTGCTTTACCGGG
SSR size	197
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	X66376
Source Species	Hordeum vulgare