GrainGenes Marker Report: HvA22S
Locus
HvA22S
Type
Microsatellite
Chromosome
7H
Map
Barley-consensus-2003-7H 67.8 Hordeum-Graner2-7H 110.2 Hordeum-Lx41-7H 76.3 Hordeum-Pillen-7H 120
[ Show Nearby Loci ]
QTL with Locus Marker
QMas.pil-7H
Map Data
Barley consensus 2003 Barley, AxHs/QTL Barley, IxF Barley, Lx41
Probe
HvA22S
Reference
Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Data Source
Langridge, Peter J.
Remark
Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe
HvA22S
Locus
HvA22S
Reference
Remarks
Insert contains repeat (t)10 Polymorphic Information Content (PIC) = 0.49 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type
SSR
PCR primers
Forward CTTCGGCTTTCCACACAACG
Reverse TTTGAAATACGGACATTTGTAAAG Left 5' CTTCGGCTTTCCACACAACG
Right 5' TTTGAAATACGGACATTTGTAAAG
SSR size
228
Amplification Conditions
High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
L19119
Source Species
Hordeum vulgare
GrainGenes Marker Report: HvA22S
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|