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GrainGenes Marker Report: HdAMYB

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Locus	HdAMYB
Type	Microsatellite
Chromosome	4H 4H + 5H
Map	Barley, B73xCPI-4H 149.5 Hordeum-Consensus2006-DArT-4H 146.9 Hordeum-Lx41-4H 203
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Map Data	Barley, B73xCPI Barley, Consensus 2006, DArT Barley, Lx41
Probe	HdAMYB
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines Number of populations contributing to the placement of HdAMYB in the Barley, Consensus 2006 DArT maps = 1
Probe	HdAMYB
Locus	HdAMYB
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (tg)10+(g)16
Type	SSR
PCR primers	GCACCAGACAGTTCACCCATA CATATATGGTCTCCCGGAAGG Left 5' GCACCAGACAGTTCACCCATA Right 5' CATATATGGTCTCCCGGAAGG
SSR size	219
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	D49999