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GrainGenes Marker Report: HVM7

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Locus	HVM7
Type	Microsatellite
Chromosome	5H
Probe	HVM7 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remark	Assigned to chromosome based on wheat-barley addition line assays.
Probe	HVM7
Locus	HVM7 HVM0007
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Saghai Maroof MA et al. (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proceedings of the National Academy of Sciences, USA 91:5466-5470.
Remarks	Insert contains repeat (AT)7.
Type	PCR SSR
PCR primers	5' ATGTAGCGGAAAAAATACCATCAT 3' 5' CCTAGCTAGTTCGTGAGCTACCTC 3' ATGTAGCGGAAAAAATACCATCAT CCTAGCTAGTTCGTGAGCTACCTC
SSR size	0.174
Amplification Conditions	PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min.
Sequence	M58753
Insert Enzyme	TaqI
Source Gene Class	Acyl carrier protein I
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.