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GrainGenes Marker Report: HVM62b

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Locus	HVM62b
Type	Microsatellite
Map	Hv-Integrated2009-5H 100.1 Hv-Nure/Tremois-5H 99.6
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Map Data	Barley, Integrated, Marcel 2009 Barley, Nure x Tremois
Probe	HVM62
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Nure x Tremois
Probe for Locus	HVM62
Locus	HVM62 HVM0062 HVM062 HVM62a HVM62b
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
General Remarks	Insert contains repeat (GA)11.
Type	PCR SSR
PCR primers	5' TCGCGACCAGACGAGAAG 3' 5' AGCTAGCCGACGACGCAC 3' TCGCGACCAGACGAGAAG AGCTAGCCGACGACGCAC
SSR size	0.251
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.