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GrainGenes Marker Report: HVM5

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Locus	HVM5
Type	Microsatellite
Synonym	Correct name HVM005 Other name HVM05 Synonym HVM005 HVM5
Chromosome	7H
Chromosome Arm	7HL
Map	Hordeum-Consensus2005-SNP-7H 149.538 [ Show all 11 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 11 ]
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	HVM5 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Probe	HVM5
Locus	HVM5 HVM005 HVM05
Reference	Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Pathogenesis-related protein Insert contains repeats (GT)6 and (AT)16.
Type	PCR SSR
PCR primers	5' AACGACGTCGCCACACAC 3' 5' AGGAACGAAGGGAGTATTAAGCAG 3'
SSR size	0.202
Amplification Conditions	PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min.
Sequence	X58566
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.