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GrainGenes Marker Report: HVM2

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Locus	HVM2
Type	Microsatellite
Synonym	Synonym HVM002B-2
Chromosome	1H
Map	Barley-consensus-2003-1H 69.1
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Map Data	Barley consensus 2003
Probe	HVM2
Data Source	Langridge, Peter J.
Probe	HVM2
Locus	HVM2a HVM2b HVM002B-1 HVM002B-2 HVM2
Reference	Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeat (GA)11.
Type	PCR SSR
PCR primers	5' CAGGTGTCTAGTGGGTGCCT 3' 5' TTACATACCAAGGAGCAATCCC 3'
SSR size	0.201
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.