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GrainGenes Marker Report: HVM034

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Locus	HVM034
Type	Microsatellite
Synonym	Other name HVM34
Map	Hordeum-Bins-6H 62
	[ Show Nearby Loci ]
Map Data	Barley, BinMap 2005
Probe	HVM34
Data Source	Kleinhofs, Andris
Remark	This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin
Probe for Locus	HVM34
Locus	HVM34 HVM034
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
General Remarks	Insert contains repeat (GA)10.
Type	PCR SSR
PCR primers	5' ACCATGTTGCGTGTTGCTT 3' 5' CGGTTCGAAATCGAGTGG 3' ACCATGTTGCGTGTTGCTT CGGTTCGAAATCGAGTGG
SSR size	0.222
Amplification Conditions	The 'touchdown' PCR comprised 38 cycles of 94oC for 1 min denaturing, and 72oC for 1 min extension. Annealing (30 sec) temperatures were progressively decreased by 1oC every second cycle from 69oC to 60oC, at which temperature they remained constant for the final 20 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.