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GrainGenes Marker Report: HVBAMY

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Locus	HVBAMY
Type	Microsatellite
Synonym	Synonym POPA3_0824 POPA3_0825
Chromosome	4H 5H
Map	Barley, B73xCPI-5H 69.5 Barley-consensus-2003-4H 103.3 Hordeum-Consensus2006-DArT-5H 79.3 Hordeum-CxB-4H 106.5 Hordeum-Pillen-4H 88
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QTL with Locus Marker	QHei.pil-4H.5 QPgw.BlKy-4H QSgw.BlKy-4H.2
Map Data	Barley consensus 2003 Barley, AxHs/QTL Barley, B73xCPI Barley, Cali-sib x BowmanBC, QTL Barley, Consensus 2006, DArT
Probe	HVBAMY
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Data Source	Langridge, Peter J.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines Number of populations contributing to the placement of HvBAMY in the Barley, Consensus 2006 DArT maps = 1
Probe	HVBAMY
Locus	HVBAMY
Synonym	Synonym HAR35_14441 HAR35_14442 HAR35_27240
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (a)17 Polymorphic Information Content (PIC) = 0.57 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	CAAGCAAAAGGTTAAAGGTGCC TTGTCGAAACCCATCCGCTC Forward CAAGCAAAAGGTTAAAGGTGCC Reverse TTGTCGAAACCCATCCGCTC Left 5' CAAGCAAAAGGTTAAAGGTGCC Right 5' TTGTCGAAACCCATCCGCTC
SSR size	132
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	D63574
Source Species	Hordeum vulgare