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GrainGenes Marker Report: H26

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Locus	H26
Type	Trait
Chromosome	3D
Chromosome Arm	3DL
Map	Wheat-Composite2004-3D 108
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	CFD211
Associated Gene	H26 (Triticum)
Gene Class	Reaction to Mayetiola destructor
Reference	Wang T et al. (2006) Genetic characterization and molecular mapping of Hessian fly resistance genes derived from Aegilops tauschii in synthetic wheat. Theoretical and Applied Genetics 113:611.
Remark	Trait marker Xcfd211-3D Nearby marker on Composite map: Xcfd211 Position on Composite map estimated by GrainGenes.
Probe for Locus	CFD211
Locus	Xcfd211-3D Xcfd211 H26
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)17 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' AGAAGACTGCACGCAAGGAT 3' Reverse 5' TGCACTAAAGCATCTTCGTGTT 3'
SSR size	283
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D
Source Species	Aegilops tauschii