GrainGenes Marker Report: Gbd
Locus
Gbd
Type
Trait
Chromosome
7D
Chromosome Arm
7DL
Map
Wheat-Composite2004-7D 215
[ Show Nearby Loci ]
Species
Triticum aestivum
Probe
WMC157
Associated Gene
Gbd (Triticum)
Gene Class
Reaction to Schizaphis graminum Rond. (Toxoptera graminum Rond.)
Reference
Zhu L et al. (2005) Inheritance and molecular mapping of new greenbug resistance genes in wheat germplasms derived from Aegilops tauschii Theoretical and Applied Genetics 111:831-837.
Remark
Trait marker Xwmc157-7D Nearby marker on Composite map: Xwmc157 Position on Composite map estimated by GrainGenes.
Probe for Locus
WMC157
Locus
Xwmc157-7D
[ Show all 6 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11c5 Donor: Rogers, Monsanto AGR204.35.SEQ. Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)29 212 to 269. Redesign primers? Bases 115-229 (ie 5' side of microsatellite) match part of 5g9. Bases 189 to 263 show homology to 24c1 bases 82 to 156 (75 in longest run, maxScore = 82), duplicate, 11c5 has precedence.
Type
PCR SSR
PCR primers
Forward cTTgATccAAgTggTTcTTTcc at 183 length 22 bases, Tm 62
Reverse TccAAATgTTTgcgAAAccTgA at 270 length 22 bases, Tm 66
SSR size
109 bases expected, Chinese Spring
Amplification Conditions
61C anneal, smear; 51C anneal, product;
Sequence
WMC157
Source Species
Triticum aestivum
GrainGenes Marker Report: Gbd
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