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GrainGenes Marker Report: GMS89

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Locus	GMS89
Type	Microsatellite
Synonym	Synonym GMS089
Chromosome	4H
Map	Barley-consensus-2003-4H 22.9 Hordeum-Pillen-4H 50
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QTL with Locus Marker	QYld.pil-4H.2
Map Data	Barley consensus 2003 Barley, AxHs/QTL
Probe	GMS89
Data Source	Langridge, Peter J.
Probe	GMS89
Locus	GMS0089 GMS089 GMS89
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Waugh R and Marshall DF (2005) Barley SSRs from the Scottish Crop Research Institute. Struss D and Plieske J (1998) The use of microsatellite markers for detection of genetic diversity in barley populations. Theoretical and Applied Genetics 97:308-315.
Remarks	SSR motif: (GA)21
Type	SSR
PCR primers	TGAAGTGGAAGGCTTCGC GCTCTCGTTGTGCGGAG
SSR size	154
Amplification Conditions	35-45 cycles: 1 min @ 94C, 1 min @ 55C, 2 min @ 72C; 1 cycle: 60 mins @ 72C.