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GrainGenes Marker Report: GMS3

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Locus
GMS3
Type
Microsatellite
Synonym
SynonymGMS003
Chromosome
2H
Map
Barley-consensus-2003-2H69.9
Hordeum-Consensus2006-DArT-2H79.6
Hordeum-Pillen-2H48
[ Show Nearby Loci ]
QTL with Locus Marker
QHea.pil-2H.2
QTgw.pil-2H.2
Map Data
Barley consensus 2003
Barley, AxHs/QTL
Barley, Consensus 2006, DArT
Probe
GMS3
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source
Langridge, Peter J.
Remark
Number of populations contributing to the placement of GMS3 in the Barley, Consensus 2006 DArT maps = 1



Probe
GMS3
Locus
GMS0003
GMS003
GMS3
Reference
ReferenceHayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferenceStruss D and Plieske J (1998) The use of microsatellite markers for detection of genetic diversity in barley populations. Theoretical and Applied Genetics 97:308-315.
Remarks
Annealing temperature = 60 deg C
Insert contains repeat (gt)15
Type
SSR
PCR primers
5' TTTCAGCATCACACGAAAGC
5' TTGCATGCATGCATACCC
Forward TTTCAGCATCACACGAAAGC
Reverse TTGCATGCATGCATACCC
TTTCAGCATCACACGAAAGC
TTGCATGCATGCATACCC
SSR size
144