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GrainGenes Marker Report: GMS27B

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Locus	GMS27B
Type	Microsatellite
Chromosome	5H
Map	Barley-consensus-2003-5H 49.9
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Map Data	Barley consensus 2003
Probe	GMS27
Data Source	Langridge, Peter J.
Probe for Locus	GMS27
Locus	GMS0027 GMS027 GMS27 GMS27A GMS27B
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Struss D and Plieske J (1998) The use of microsatellite markers for detection of genetic diversity in barley populations. Theoretical and Applied Genetics 97:308-315.
General Remarks	Annealing temperature = 60 deg C Insert contains repeat (gt)5(ct)2(gt)27
Type	SSR
PCR primers	5' CTTTTTCTTTGACGATGCACC 5' TGAGTTTGTGAGAACTGGATGG CTTTTTCTTTGACGATGCACC TGAGTTTGTGAGAACTGGATGG
SSR size	154