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GrainGenes Marker Report: GMS0061

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Locus	GMS0061
Type	Microsatellite
Map	Barley, B73xCPI-5H 120.1
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Map Data	Barley, B73xCPI
Probe	GMS61
Probe for Locus	GMS61
Locus	GMS0061 GMS061
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Struss D and Plieske J (1998) The use of microsatellite markers for detection of genetic diversity in barley populations. Theoretical and Applied Genetics 97:308-315.
General Remarks	Annealing temperature = 60 deg C Insert contains repeat (ga)16
Type	SSR
PCR primers	5' CACCTGTTCCGTCCCGTC 5' AACCTCTTTTTTATCCCTCGC CACCTGTTCCGTCCCGTC AACCTCTTTTTTATCCCTCGC Forward CACCTGTTCCGTCCCGTC Reverse AACCTCTTTTTTATCCCTCGC
SSR size	145