GrainGenes Marker Report: GBS0560-3H
Locus
GBS0560-3H
Type
SNP
Map
Hv-Consensus2006-Stein-3H 150.2 Hv-SxM-Stein-3H 125.91
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein Barley, SxM, Stein2006
Probe
GBS560
Probe for Locus
GBS560
Locus
GBS0560 GBS0560-3H
Reference
Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks
Clone HM12P03r
PCR primers
AATCCCACTGCAGCAACAAA
CACCCAACACGCATCAGTCA
Amplification Conditions
5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BU996231
DNA Library
IPK-HM
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBS0560-3H
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